tyrode s buffer Search Results


heparinized tyrodes buffer  (Eppendorf AG)
90
Eppendorf AG heparinized tyrodes buffer
Heparinized Tyrodes Buffer, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/heparinized tyrodes buffer/product/Eppendorf AG
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heparinized tyrodes buffer - by Bioz Stars, 2026-03
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modified tyrodes-hepes buffer containing 2 mm mg 2+  (Corning Life Sciences)
90
Corning Life Sciences modified tyrodes-hepes buffer containing 2 mm mg 2+
Washed platelets were resuspended at a concentration of 4 × 108/mL in <t>Tyrodes</t> buffer containing 2 mM MgCl2 and 0.5 mM CaCl2 and added to culture plates that had been pre-coated with 50 μg/mL of laminin 511. Platelets were allowed to settle, bind, and spread at 37°C for the indicated times, and then detergent lysed and subjected to immunoprecipitation analysis using mAbs PECAM-1.3 for human platelets and 390 for murine platelets. Western blots were developed using the anti-phosphotyrosine mAb PY20 (top panels) and anti-PECAM-1 polyclonal antibodies SEW32-34 (human) and M-20 (mouse) to visualize antigen loading. Maximal phosphorylation of PECAM-1 at the 45 minute time point reflects the time needed for platelets to settle, make contact with the immobilized laminin, and activate the platelets. The result shown is representative of three separate experiments.
Modified Tyrodes Hepes Buffer Containing 2 Mm Mg 2+, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/modified tyrodes-hepes buffer containing 2 mm mg 2+/product/Corning Life Sciences
Average 90 stars, based on 1 article reviews
modified tyrodes-hepes buffer containing 2 mm mg 2+ - by Bioz Stars, 2026-03
90/100 stars
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tyrode's buffer  (Blackwell Science Ltd)
90
Blackwell Science Ltd tyrode's buffer
Washed platelets were resuspended at a concentration of 4 × 108/mL in <t>Tyrodes</t> buffer containing 2 mM MgCl2 and 0.5 mM CaCl2 and added to culture plates that had been pre-coated with 50 μg/mL of laminin 511. Platelets were allowed to settle, bind, and spread at 37°C for the indicated times, and then detergent lysed and subjected to immunoprecipitation analysis using mAbs PECAM-1.3 for human platelets and 390 for murine platelets. Western blots were developed using the anti-phosphotyrosine mAb PY20 (top panels) and anti-PECAM-1 polyclonal antibodies SEW32-34 (human) and M-20 (mouse) to visualize antigen loading. Maximal phosphorylation of PECAM-1 at the 45 minute time point reflects the time needed for platelets to settle, make contact with the immobilized laminin, and activate the platelets. The result shown is representative of three separate experiments.
Tyrode's Buffer, supplied by Blackwell Science Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/tyrode's buffer/product/Blackwell Science Ltd
Average 90 stars, based on 1 article reviews
tyrode's buffer - by Bioz Stars, 2026-03
90/100 stars
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tyrode's buffer  (Agar Scientific)
90
Agar Scientific tyrode's buffer
Washed platelets were resuspended at a concentration of 4 × 108/mL in <t>Tyrodes</t> buffer containing 2 mM MgCl2 and 0.5 mM CaCl2 and added to culture plates that had been pre-coated with 50 μg/mL of laminin 511. Platelets were allowed to settle, bind, and spread at 37°C for the indicated times, and then detergent lysed and subjected to immunoprecipitation analysis using mAbs PECAM-1.3 for human platelets and 390 for murine platelets. Western blots were developed using the anti-phosphotyrosine mAb PY20 (top panels) and anti-PECAM-1 polyclonal antibodies SEW32-34 (human) and M-20 (mouse) to visualize antigen loading. Maximal phosphorylation of PECAM-1 at the 45 minute time point reflects the time needed for platelets to settle, make contact with the immobilized laminin, and activate the platelets. The result shown is representative of three separate experiments.
Tyrode's Buffer, supplied by Agar Scientific, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/tyrode's buffer/product/Agar Scientific
Average 90 stars, based on 1 article reviews
tyrode's buffer - by Bioz Stars, 2026-03
90/100 stars
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tyrode's albumin buffer containing 10 u/ml heparin and 0.5 μmol/l pgi  (DUTSCHER DOMINIQUE)
90
DUTSCHER DOMINIQUE tyrode's albumin buffer containing 10 u/ml heparin and 0.5 μmol/l pgi
Washed platelets were resuspended at a concentration of 4 × 108/mL in <t>Tyrodes</t> buffer containing 2 mM MgCl2 and 0.5 mM CaCl2 and added to culture plates that had been pre-coated with 50 μg/mL of laminin 511. Platelets were allowed to settle, bind, and spread at 37°C for the indicated times, and then detergent lysed and subjected to immunoprecipitation analysis using mAbs PECAM-1.3 for human platelets and 390 for murine platelets. Western blots were developed using the anti-phosphotyrosine mAb PY20 (top panels) and anti-PECAM-1 polyclonal antibodies SEW32-34 (human) and M-20 (mouse) to visualize antigen loading. Maximal phosphorylation of PECAM-1 at the 45 minute time point reflects the time needed for platelets to settle, make contact with the immobilized laminin, and activate the platelets. The result shown is representative of three separate experiments.
Tyrode's Albumin Buffer Containing 10 U/Ml Heparin And 0.5 μmol/L Pgi, supplied by DUTSCHER DOMINIQUE, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/tyrode's albumin buffer containing 10 u/ml heparin and 0.5 μmol/l pgi/product/DUTSCHER DOMINIQUE
Average 90 stars, based on 1 article reviews
tyrode's albumin buffer containing 10 u/ml heparin and 0.5 μmol/l pgi - by Bioz Stars, 2026-03
90/100 stars
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tyrode's buffer  (HiMedia Laboratories)
90
HiMedia Laboratories tyrode's buffer
Washed platelets were resuspended at a concentration of 4 × 108/mL in <t>Tyrodes</t> buffer containing 2 mM MgCl2 and 0.5 mM CaCl2 and added to culture plates that had been pre-coated with 50 μg/mL of laminin 511. Platelets were allowed to settle, bind, and spread at 37°C for the indicated times, and then detergent lysed and subjected to immunoprecipitation analysis using mAbs PECAM-1.3 for human platelets and 390 for murine platelets. Western blots were developed using the anti-phosphotyrosine mAb PY20 (top panels) and anti-PECAM-1 polyclonal antibodies SEW32-34 (human) and M-20 (mouse) to visualize antigen loading. Maximal phosphorylation of PECAM-1 at the 45 minute time point reflects the time needed for platelets to settle, make contact with the immobilized laminin, and activate the platelets. The result shown is representative of three separate experiments.
Tyrode's Buffer, supplied by HiMedia Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/tyrode's buffer/product/HiMedia Laboratories
Average 90 stars, based on 1 article reviews
tyrode's buffer - by Bioz Stars, 2026-03
90/100 stars
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heparinized murine tyrode's buffer  (Eppendorf AG)
90
Eppendorf AG heparinized murine tyrode's buffer
Washed platelets were resuspended at a concentration of 4 × 108/mL in <t>Tyrodes</t> buffer containing 2 mM MgCl2 and 0.5 mM CaCl2 and added to culture plates that had been pre-coated with 50 μg/mL of laminin 511. Platelets were allowed to settle, bind, and spread at 37°C for the indicated times, and then detergent lysed and subjected to immunoprecipitation analysis using mAbs PECAM-1.3 for human platelets and 390 for murine platelets. Western blots were developed using the anti-phosphotyrosine mAb PY20 (top panels) and anti-PECAM-1 polyclonal antibodies SEW32-34 (human) and M-20 (mouse) to visualize antigen loading. Maximal phosphorylation of PECAM-1 at the 45 minute time point reflects the time needed for platelets to settle, make contact with the immobilized laminin, and activate the platelets. The result shown is representative of three separate experiments.
Heparinized Murine Tyrode's Buffer, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/heparinized murine tyrode's buffer/product/Eppendorf AG
Average 90 stars, based on 1 article reviews
heparinized murine tyrode's buffer - by Bioz Stars, 2026-03
90/100 stars
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hepes modified tyrode's albumin (hmta) buffer cellphire rgt-004  (Cellphire Inc)
90
Cellphire Inc hepes modified tyrode's albumin (hmta) buffer cellphire rgt-004
Washed platelets were resuspended at a concentration of 4 × 108/mL in <t>Tyrodes</t> buffer containing 2 mM MgCl2 and 0.5 mM CaCl2 and added to culture plates that had been pre-coated with 50 μg/mL of laminin 511. Platelets were allowed to settle, bind, and spread at 37°C for the indicated times, and then detergent lysed and subjected to immunoprecipitation analysis using mAbs PECAM-1.3 for human platelets and 390 for murine platelets. Western blots were developed using the anti-phosphotyrosine mAb PY20 (top panels) and anti-PECAM-1 polyclonal antibodies SEW32-34 (human) and M-20 (mouse) to visualize antigen loading. Maximal phosphorylation of PECAM-1 at the 45 minute time point reflects the time needed for platelets to settle, make contact with the immobilized laminin, and activate the platelets. The result shown is representative of three separate experiments.
Hepes Modified Tyrode's Albumin (Hmta) Buffer Cellphire Rgt 004, supplied by Cellphire Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/hepes modified tyrode's albumin (hmta) buffer cellphire rgt-004/product/Cellphire Inc
Average 90 stars, based on 1 article reviews
hepes modified tyrode's albumin (hmta) buffer cellphire rgt-004 - by Bioz Stars, 2026-03
90/100 stars
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tyrodes facs buffer  (Becton Dickinson)
90
Becton Dickinson tyrodes facs buffer
Washed platelets were resuspended at a concentration of 4 × 108/mL in <t>Tyrodes</t> buffer containing 2 mM MgCl2 and 0.5 mM CaCl2 and added to culture plates that had been pre-coated with 50 μg/mL of laminin 511. Platelets were allowed to settle, bind, and spread at 37°C for the indicated times, and then detergent lysed and subjected to immunoprecipitation analysis using mAbs PECAM-1.3 for human platelets and 390 for murine platelets. Western blots were developed using the anti-phosphotyrosine mAb PY20 (top panels) and anti-PECAM-1 polyclonal antibodies SEW32-34 (human) and M-20 (mouse) to visualize antigen loading. Maximal phosphorylation of PECAM-1 at the 45 minute time point reflects the time needed for platelets to settle, make contact with the immobilized laminin, and activate the platelets. The result shown is representative of three separate experiments.
Tyrodes Facs Buffer, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/tyrodes facs buffer/product/Becton Dickinson
Average 90 stars, based on 1 article reviews
tyrodes facs buffer - by Bioz Stars, 2026-03
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tyrode's buffer  (Corning Life Sciences)
90
Corning Life Sciences tyrode's buffer
Washed platelets were resuspended at a concentration of 4 × 108/mL in <t>Tyrodes</t> buffer containing 2 mM MgCl2 and 0.5 mM CaCl2 and added to culture plates that had been pre-coated with 50 μg/mL of laminin 511. Platelets were allowed to settle, bind, and spread at 37°C for the indicated times, and then detergent lysed and subjected to immunoprecipitation analysis using mAbs PECAM-1.3 for human platelets and 390 for murine platelets. Western blots were developed using the anti-phosphotyrosine mAb PY20 (top panels) and anti-PECAM-1 polyclonal antibodies SEW32-34 (human) and M-20 (mouse) to visualize antigen loading. Maximal phosphorylation of PECAM-1 at the 45 minute time point reflects the time needed for platelets to settle, make contact with the immobilized laminin, and activate the platelets. The result shown is representative of three separate experiments.
Tyrode's Buffer, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/tyrode's buffer/product/Corning Life Sciences
Average 90 stars, based on 1 article reviews
tyrode's buffer - by Bioz Stars, 2026-03
90/100 stars
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tyrode's buffer  (Cadisch MDA)
90
Cadisch MDA tyrode's buffer
Washed platelets were resuspended at a concentration of 4 × 108/mL in <t>Tyrodes</t> buffer containing 2 mM MgCl2 and 0.5 mM CaCl2 and added to culture plates that had been pre-coated with 50 μg/mL of laminin 511. Platelets were allowed to settle, bind, and spread at 37°C for the indicated times, and then detergent lysed and subjected to immunoprecipitation analysis using mAbs PECAM-1.3 for human platelets and 390 for murine platelets. Western blots were developed using the anti-phosphotyrosine mAb PY20 (top panels) and anti-PECAM-1 polyclonal antibodies SEW32-34 (human) and M-20 (mouse) to visualize antigen loading. Maximal phosphorylation of PECAM-1 at the 45 minute time point reflects the time needed for platelets to settle, make contact with the immobilized laminin, and activate the platelets. The result shown is representative of three separate experiments.
Tyrode's Buffer, supplied by Cadisch MDA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/tyrode's buffer/product/Cadisch MDA
Average 90 stars, based on 1 article reviews
tyrode's buffer - by Bioz Stars, 2026-03
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tyrode's buffer containing prostaglandin e1 (1 μm final concentration)  (Cayman Chemical)
90
Cayman Chemical tyrode's buffer containing prostaglandin e1 (1 μm final concentration)
Washed platelets were resuspended at a concentration of 4 × 108/mL in <t>Tyrodes</t> buffer containing 2 mM MgCl2 and 0.5 mM CaCl2 and added to culture plates that had been pre-coated with 50 μg/mL of laminin 511. Platelets were allowed to settle, bind, and spread at 37°C for the indicated times, and then detergent lysed and subjected to immunoprecipitation analysis using mAbs PECAM-1.3 for human platelets and 390 for murine platelets. Western blots were developed using the anti-phosphotyrosine mAb PY20 (top panels) and anti-PECAM-1 polyclonal antibodies SEW32-34 (human) and M-20 (mouse) to visualize antigen loading. Maximal phosphorylation of PECAM-1 at the 45 minute time point reflects the time needed for platelets to settle, make contact with the immobilized laminin, and activate the platelets. The result shown is representative of three separate experiments.
Tyrode's Buffer Containing Prostaglandin E1 (1 μm Final Concentration), supplied by Cayman Chemical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/tyrode's buffer containing prostaglandin e1 (1 μm final concentration)/product/Cayman Chemical
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tyrode's buffer containing prostaglandin e1 (1 μm final concentration) - by Bioz Stars, 2026-03
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Image Search Results


Washed platelets were resuspended at a concentration of 4 × 108/mL in Tyrodes buffer containing 2 mM MgCl2 and 0.5 mM CaCl2 and added to culture plates that had been pre-coated with 50 μg/mL of laminin 511. Platelets were allowed to settle, bind, and spread at 37°C for the indicated times, and then detergent lysed and subjected to immunoprecipitation analysis using mAbs PECAM-1.3 for human platelets and 390 for murine platelets. Western blots were developed using the anti-phosphotyrosine mAb PY20 (top panels) and anti-PECAM-1 polyclonal antibodies SEW32-34 (human) and M-20 (mouse) to visualize antigen loading. Maximal phosphorylation of PECAM-1 at the 45 minute time point reflects the time needed for platelets to settle, make contact with the immobilized laminin, and activate the platelets. The result shown is representative of three separate experiments.

Journal:

Article Title: PECAM-1 is a negative regulator of laminin-induced platelet activation

doi: 10.1111/j.1538-7836.2010.03883.x

Figure Lengend Snippet: Washed platelets were resuspended at a concentration of 4 × 108/mL in Tyrodes buffer containing 2 mM MgCl2 and 0.5 mM CaCl2 and added to culture plates that had been pre-coated with 50 μg/mL of laminin 511. Platelets were allowed to settle, bind, and spread at 37°C for the indicated times, and then detergent lysed and subjected to immunoprecipitation analysis using mAbs PECAM-1.3 for human platelets and 390 for murine platelets. Western blots were developed using the anti-phosphotyrosine mAb PY20 (top panels) and anti-PECAM-1 polyclonal antibodies SEW32-34 (human) and M-20 (mouse) to visualize antigen loading. Maximal phosphorylation of PECAM-1 at the 45 minute time point reflects the time needed for platelets to settle, make contact with the immobilized laminin, and activate the platelets. The result shown is representative of three separate experiments.

Article Snippet: 700 μl of washed human or murine platelets (6 × 10 8 /mL) suspended in modified Tyrodes-HEPES buffer containing 2 mM Mg 2+ were seeded onto laminin or collagen-coated Corning cell culture dishes (Corning Inc., Corning, NY) and incubated at 37 °C for the indicated time period.

Techniques: Concentration Assay, Immunoprecipitation, Western Blot